Preparation and characterization of bFGF and BSA loaded microspheres

ÇETİN M., ÇAPAN Y., VURAL İ., DOĞAN A. L., GÜÇ D., Hıncal A., ...Daha Fazla

Journal Of Drug Delivery Science And Technology, cilt.15, sa.5, ss.371-375, 2005 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 15 Sayı: 5
  • Basım Tarihi: 2005
  • Doi Numarası: 10.1016/s1773-2247(05)50067-4
  • Dergi Adı: Journal Of Drug Delivery Science And Technology
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.371-375
  • Anahtar Kelimeler: PLGA, microspheres, BSA, bFGF, SDS-PAGE, FIBROBLAST-GROWTH-FACTOR, BLOOD-BRAIN-BARRIER, POLY(LACTIDE-CO-GLYCOLIDE) MICROSPHERES, DELIVERY-SYSTEM, RELEASE, PROTEIN, NANOPARTICLES, NANOSPHERES, GLYCOLIDE, ISCHEMIA
  • Atatürk Üniversitesi Adresli: Hayır

Özet

This article describes the preparation and characterization of BSA and bFGF-loaded PLGA microspheres and assesses the effect of entrapment procedure and in vitro release conditions. In the experiments, changing the PVA concentration from 3 to 5% (w/v) in the pre-emulsion innerphase led to a change in particle size and encapsulation efficiency of BSA-loaded and bFGF-loaded PLGA microspheres from 1 to 0.7 mu m and from 73 to 65%, respectively. The in vitro release of the bFGF from PLGA microspheres was much lower than BSA-loaded PLGA microspheres. For all of the formulations, BSA and bFGF retained integrity after the microencapsulation process as evaluated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Besides, the cell proliferation-stimulating activity of bFGF was evaluated by using BALB/c 3T3 cells and it was shown that the bioactivity of bFGF was increased by adding heparin to the release medium.