Extraction of DNA from soil using nanoparticles by magnetic bioseparation
Letters in Applied Microbiology, cilt.46, sa.4, ss.488-491, 2008 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 46 Sayı: 4
- Basım Tarihi: 2008
- Doi Numarası: 10.1111/j.1472-765x.2008.02343.x
- Dergi Adı: Letters in Applied Microbiology
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.488-491
- Anahtar Kelimeler: DNA, magnetic bioseparation, PCR, rapid techniques, soil
- Atatürk Üniversitesi Adresli: Hayır
Özet
Aims: To develop a simple, rapid and inexpensive soil DNA extraction protocol. Methods and Results: The protocol relies on the use of superparamagnetic silica-magnetite nanoparticles for the isolation and purification of DNA from soil samples. DNA suitable for use in molecular biology applications was obtained from a number of soil samples. Conclusions: The DNA extracted using the tested method successfully permitted the PCR amplification of a fragment of the bacterial 16S rDNA gene. The extracted DNA could also be restriction endonuclease digested. Significance and Impact of the Study: The protocol reported here is simple and permits rapid isolation of PCR-ready soil DNA. The method requires only small quantities of soil sample, is scalable and suitable for automation. © 2008 The Authors.