Akademik Veri Yönetim Sistemi
PHYTOPHARMACOLOGY AND THERAPEUTIC VALUES V, cilt.23, ss.325-335, 2009 (Hakemli Dergi)
Proteases perform very important functions and are used in different objectives as in vitro. In recent years, proteases have been used for both clinical and pharmaceutical (good digestion, anti-inflammatory, etc.) and industrial applications (cheese production, softening meat, leather trading). News of diseases passing over from animals to human (sars, mad cow, bird flu) took place so much in the press that the suspicion of contamination in food production increased. In this research, a protease (melain N) enzyme was purified and characterized from green fruits of tasbih agaji. This plant grows abundantly in certain regions of Turkey. (NH)(2)SO4 fractionation and CM-sephadex ion exchange chromatography methods were used in the purification of the protease. Optimum pH value was determined as 8 and the optimum temperature was determined as 70 degrees C. The V-max and K-M values were determined by means of Linewearver-Burk graphics as 0.572 g/L. min and 0.032 g/L, respectively. The purification degree was controlled by using a SDS-PAGE and molecular weight its four-subunit was determined as 25kD. Then, the molecular weight of the enzyme was determined as 25 kD by using the gel filtration chromatograph. Because of the enzyme was inhibited by iodoacetic acid and Hg2+ but was not inhibited by phenylmethanesulphonyl fluoride or EDTA, it was named cysteine protease.