Evaluation of computational modifications in HPTLC with Gel analysis software and flatbed scanner for lipid separation

Kaynar Ö., İleritürk M., Hayırlı A.

Journal of Planar Chromatography - Modern TLC, cilt.26, ss.202-208, 2013 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 26
  • Basım Tarihi: 2013
  • Doi Numarası: 10.1556/jpc.26.2013.3.1
  • Dergi Adı: Journal of Planar Chromatography - Modern TLC
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.202-208
  • Anahtar Kelimeler: TL 120 software, Flatbed scanner, Visible light, High quality image
  • Atatürk Üniversitesi Adresli: Evet

Özet

High-performance thin-layer chromatography plays an important role in the determination of lipid fractions. However, it requires expensive equipment for quantification of lipids spots. In this experiment, flatbed scanner with gel analysis software was employed to determine lipid fractions of egg yolk, with testing some modifications: optical resolution (300 vs. 600 dpi), lane selection method (manual vs. automatic), and background subtraction options (none, lane edge, minimum profile, rubber band, rolling ball, or valley to valley). Data show that using flatbed scanner with gel analysis software is economically feasible and yields reliable and reproducible results. The best chromatogram was achieved at 600 dpi resolution with no background subtraction, regardless of lane selection.