Effect of Danofloxacin Treatment on the Development of Fluoroquinolone Resistance in Campylobacter jejuni in Calves

Goulart D. B., Beyi A. F., Wu Z., Adiguzel M. C., Schroeder A., Singh K., ...Daha Fazla

ANTIBIOTICS-BASEL, cilt.11, sa.4, 2022 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 11 Sayı: 4
  • Basım Tarihi: 2022
  • Doi Numarası: 10.3390/antibiotics11040531
  • Dergi Adı: ANTIBIOTICS-BASEL
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, CAB Abstracts, Chemical Abstracts Core, EMBASE, Veterinary Science Database, Directory of Open Access Journals
  • Anahtar Kelimeler: Campylobacter, cattle, colonization, rectal feces, bovine respiratory disease (BRD), fluoroquinolone, danofloxacin treatment, antimicrobial resistance, pulsed-field gel electrophoresis, minimum inhibitory concentration, BOVINE RESPIRATORY-DISEASE, COMMERCIAL POULTRY FLOCKS, ANTIMICROBIAL RESISTANCE, THERMOPHILIC CAMPYLOBACTERS, FEEDLOT CATTLE, DAIRY-CATTLE, CIPROFLOXACIN RESISTANCE, MOLECULAR EPIDEMIOLOGY, ESCHERICHIA-COLI, STRAIN DIVERSITY
  • Atatürk Üniversitesi Adresli: Evet

Özet

Campylobacter is a leading cause of foodborne gastroenteritis. Recent studies have indicated a rise in fluoroquinolone-resistant (FQ-R) Campylobacter in cattle, where FQ is used to control bovine respiratory disease (BRD). To assess the effect of danofloxacin treatment on the development of FQ-resistance in C. jejuni, 30 commercial calves were divided into Group 1, Group 2, and Group 3 (n = 10), and were all inoculated orally with FQ-susceptible (FQ-S) C. jejuni; seven days later, Group 3 was challenged with transtracheal Mannheimia haemolytica, and one week later, Group 2 and Group 3 were injected subcutaneously with danofloxacin. Rectal feces were collected to determine relative percentages of FQ-R Campylobacter via culture. Before oral inoculation with C. jejuni, 87% of calves were naturally colonized by FQ-R C. jejuni. Two days after the inoculation, FQ-R C. jejuni decreased substantially in the majority of calves. Within 24 h of danofloxacin injection, almost all C. jejuni populations shifted to an FQ-R phenotype in both FQ-treated groups, which was only transitory, as FQ-S strains became predominant during later periods. Genotyping indicated that the spike seen in FQ-R C. jejuni populations following the injection was due mainly to enrichment of preexisting FQ-R C. jejuni, rather than development of de novo FQ resistance in susceptible strains. These results provide important insights into the dynamic changes of FQ-resistant Campylobacter in cattle in response to FQ treatment.