Akademik Veri Yönetim Sistemi
52nd International Symposium on Essential Oils, Wroclaw, Polonya, 4 - 07 Eylül 2022, ss.1-2
Objective
Prangos platychlaena subsp. platychloena is found in the East and North East Anatolian Regions of Turkey. In traditional medicine, the dried and crushed roots are used externally for wound healing in animals. Here we report on the antimicrobial activity of the essential oils from Prangos platychlaena subsp. platychloena because very little information is available on these endemic perennial herbaceous plants.
Methods
The aerial parts of Prangos platychlaena subsp. platychloena were collected at the flowering stage in August 2021 in the vicinity of Erzurum (altitude 2100 m, 40°20'21.6"N, 40°48'51.4"E), Turkey. The aerial parts of air-dried plant material (200 g) were subjected to hydrodistillation using a Clevenger-type apparatus for 3 hours. The obtained essential oil was stored at +4 oC until tested and analyzed. The essential oil was tested individually against a range of 42 microorganisms, including 29 bacteria, 10 mold, and 3 yeast species. Antimicrobial tests were carried out by the disk diffusion method [1, 2] using 100 µL of suspension containing 108 colony forming units (CFU)/mL of bacteria, 106 CFU/mL of yeast, and 104 spores/mL of mold spread on Nutrient Agar (NA), Sabouraud Dextrose Agar (SDA), and potato dextrose agar (PDA) medium, respectively. The MIC values were determined for the bacterial and yeast strains that were sensitive to the essential oil in the microwell dilution assay. The agar dilution method was used to determine the MIC values of the fungus strains. The inoculation of the strains was prepared from fresh broth cultures, and suspensions were adjusted to 0.5 McFarland standard turbidity (BioSan DEN-1 McFarland Densitometer). The MIC values of prepared serial the essential oil dilutions between 7,8 to 500 µg/mL were determined on the basis of a microwell dilution method. At the end of the incubation period, the plates were evaluated for the presence or absence of growth. Each test was repeated at least triplicate.
Results
Essential oil yield (1.92 %) (v/w) of Prangos platychlaena subsp. platychloena was calculated on the basis of dry weight of the plant material. The main components of the oil were α-bisabolol (21.51 %). It was determined that this essential oil showed an antagonistic effect on all strains. Bacillus subtilis, Listeria monocytogenes, and S. aureus were the most affected bacterial strains. It has also been observed that the development of pathogenic, spoiling and toxic strains such as Escherichia coli, Salmonella Typhimurium, Proteus spp., Pseudomonas spp., Candida albicans, and Aspergillus flavus can be inhibited. Especially, the antifungal effect, which shows this essential oil on mold, is remarkable in terms of mildew development and prevention of mycotoxin formation.
Conclusions
All the above results demonstrated that Prangos platychlaena subsp. platychloena oil had the potential to control foodborne microorganisms and therefore could be used as a preservative for food products. However, care should be taken in the use in foods containing probiotic bacteria such as kefir and prebiotic yogurt, because of the possible adverse effects of Prangos platychlaena subsp. platychloena oil on probiotics.