Analysis of Genetic Relationships of Mulberry (Morus L.) Genotypes Using Inter-Simple Sequence Repeats (ISSR) Markers

Keskin S., AKÇA Y., ERCİŞLİ S.

ERWERBS-OBSTBAU, cilt.64, sa.1, ss.75-83, 2022 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 64 Sayı: 1
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1007/s10341-021-00614-1
  • Dergi Adı: ERWERBS-OBSTBAU
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Agricultural & Environmental Science Database, CAB Abstracts, Environment Index, Food Science & Technology Abstracts
  • Sayfa Sayıları: ss.75-83
  • Anahtar Kelimeler: Morus alba, Genetic diversitiy, Molecular markers, Mulberry, MOLECULAR CHARACTERIZATION, SPP., RAPD, COLLECTION, RESOURCES, DIVERSITY, VARIABILITY, ACCESSIONS, CULTIVARS, VARIETIES
  • Atatürk Üniversitesi Adresli: Evet

Özet

In this study, inter-simple sequence repeats (ISSR) markers were used to investigate genetic relationships of 83 mulberry (Morus alba L.) genotypes being located at 38 degrees 39 ' 45 ' 40 ' 50 ' N latitude and 45 ' 40 degrees 12 ' E longitude in Turkey. Eight primer combinations generated a total of 106 bands, 95 of which were polymorphic. The polymorphism rate of the primers was found to be 89.5% on average. The highest polymorphism rate was obtained from the primer no. 807 (100%) and lowest rate was obtained from the primer 841 (80%). The number of bands obtained per primer ranged from 7 (ISSR 807) to 18 (ISSR 835), and the mean band number was found to be 13.25. The mean genetic similarity coefficients among all mulberry genotypes ranged between 0.54 and 1.00. In genotypes, the highest genetic similarity value was found in TO25 and TO39 genotypes (1.00). An UPGMA cluster of the 83 mulberry genotypes constructed from ISSR fragment Nei's similarities separated 83 mulberry genotypes into four major clusters groups. The results from the cluster analysis were in general agreement with our morphologic classification. In addition, the fact that genotypes have vast genetic variation in a narrow area showed that these genotypes can be used in future mulberry breeding programs.