Akademik Veri Yönetim Sistemi
Pharmata, cilt.3, sa.3, ss.59-63, 2023 (Hakemli Dergi)
Objective: The development and validation of a novel, simple, and quick high-performance liquid
chromatography–ultraviolet detection (HPLC-UV) technique for measuring meloxicam in pharmaceutical formulations was made.
Methods: The technique parameters were tuned to be 0.8 mL/min flow rate, variable column
temperature, 290 nm wavelength, 10 µL injection volume, and a mobile phase combination of
water (with 0.6% triflour acetic acid—pH:2.6) and methanol (30 : 70 v/v) to carry out this study. In
this study, valsartan was used as ınternal standard (IS).
Results: Specificity, the limit of quantitation (LOQ), linearity, accuracy, precision, stability, recovery, and ruggedness were all tested. The technique was linear between 1.0 µg/mL and 50 µg/mL,
with precision (relative standard d (RSD) %) and accuracy (relative error %) of less than 3.9% and
0.7%, respectively. The LOQ and LOD values of method were 1.00 and 0.25 µg/mL, respectively.
Analytical recovery from pharmaceutical preparations was performed according to the standard
addition method, and the average analytical recovery value was determined as 100.4%. The developed and validated HPLC-UV method was successfully applied to 4 commercial tablet dosage
formulations obtained from a local pharmacy store in Turkey (Zeloxim, Melox, Meksun, Exen).
Conclusion: It has been concluded that the developed HPLC-UV method is sensitive, accurate,
and precise and can be successfully applied in quality control studies in the pharmaceutical
industry.