Molecular Typing of Thermophilic Bacilli isolated from Different Hot Springs of Turkey

YANMIS D., ADIGÜZEL A.

RESEARCH JOURNAL OF BIOTECHNOLOGY, cilt.9, sa.10, ss.83-88, 2014 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 9 Sayı: 10
  • Basım Tarihi: 2014
  • Dergi Adı: RESEARCH JOURNAL OF BIOTECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.83-88
  • Anahtar Kelimeler: Thermophilic bacteria, FAMEs, rep-PCR, 16S rRNA sequencing, RETAIL BEEF, DEGREES-C, GEOBACILLUS, ERZURUM, STEAROTHERMOPHILUS, DIVERSITY, STRAINS, PCR
  • Atatürk Üniversitesi Adresli: Evet

Özet

In this study, the molecular characterization of 10 thermophilic bacilli isolated from different geothermal areas in Turkey was done by using the fatty acid methyl ester, 16S rRNA sequencing and rep-PCR profiling methods. Depending upon the data obtained from fatty acid analysis, it was determined that there were 8 different fatty acids in the examined 10 bacterial strains and 16:0 and 17:0 anteiso were determined to be the fundamental fatty acid. In consequence of the comparison of 16SrRNA sequence analysis results with the data in GenBank, all the isolates were identified to resemble Aeribacillus pallidus at a rate of >= 98 %. Later on, rep-PCR was performed for the purpose of determining the differences between these isolates.