A competitive immunoassay for detecting triazophos based on fluorescent catalytic hairpin self-assembly

Wang Y., Abd El-Aty A. M. A., Chen G., Jia H., Cui X., Xu L., ...Daha Fazla

MICROCHIMICA ACTA, cilt.189, sa.3, 2022 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 189 Sayı: 3
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1007/s00604-022-05217-5
  • Dergi Adı: MICROCHIMICA ACTA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Analytical Abstracts, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), Biotechnology Research Abstracts, Chemical Abstracts Core, Chimica, Communication Abstracts, EMBASE, Food Science & Technology Abstracts, MEDLINE, Metadex, Pollution Abstracts, Civil Engineering Abstracts
  • Anahtar Kelimeler: Catalyzed hairpin self-assembly, Enzyme-free amplification, Fluorescence detection, Triazophos, Gold nanoparticles, ORGANOPHOSPHORUS PESTICIDES, ELECTROCHEMICAL DETECTION, ULTRASENSITIVE DETECTION, BIO-BARCODE, ASSAY
  • Atatürk Üniversitesi Adresli: Evet

Özet

A rapid detection method is introduced for residual trace levels of triazophos in water and agricultural products using an immunoassay based on catalytic hairpin self-assembly (CHA). The gold nanoparticle (AuNPs) surface was modified with triazophos antibody and sulfhydryl bio-barcode, and an immune competition reaction system was established between triazophos and its ovalbumin-hapten (OVA-hapten). The bio-barcode served as a catalyst to continuously induce the CHA reaction to achieve the dual signal amplification. The method does not rely on the participation of enzymes, and the addition of fluorescent materials in the last step avoids interfering factors, such as a fluorescence burst. The emitted fluorescence was detected at 489/521 nm excitation/emission wavelengths. The detection range of the developed method was 0.01-50 ng/mL for triazophos, and the limit of detection (LOD) was 0.0048 ng/mL. The developed method correlates well with the results obtained by LC-MS/MS, with satisfactory recovery and sensitivity. In sum, the designed method is reliable and provides a new approach to detect pesticide residues rapidly and quantitatively.