Antioxidant Activity and Phytochemical Profiling of Steam-Distilled Oil of Flaxseed (Linum usitatissimum): Therapeutic Targeting Against Glaucoma, Oxidative Stress, Cholinergic Imbalance, and Diabetes

GÜLÇİN İ., Mutlu M., Bingol Z., Ozden E. M., Mirzaee Z., Goren A. C., ...Daha Fazla

Molecules, cilt.30, sa.16, 2025 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 30 Sayı: 16
  • Basım Tarihi: 2025
  • Doi Numarası: 10.3390/molecules30163384
  • Dergi Adı: Molecules
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Communication Abstracts, Food Science & Technology Abstracts, MEDLINE, Metadex, Veterinary Science Database, Directory of Open Access Journals, Civil Engineering Abstracts
  • Anahtar Kelimeler: GC/MS, LC-HRMS, Linum usitatissimum, metabolic disorders, radical scavenging
  • Atatürk Üniversitesi Adresli: Evet

Özet

This investigation explored the chemical constituents and biological activities of the steam-distilled oil of L. usitatissimum (SDOLU), employing sophisticated techniques including LC-HRMS, GC-MS, and GC-FID. The analysis identified a diverse array of 17 phenolic compounds, with linoleoyl chloride (64.05%) and linoleic acid (10.39%) as the major fatty acid components. The SDOLU demonstrated remarkable antioxidant capacity, effectively neutralizing free radicals in both DPPH• (IC50: 19.80 μg/mL) and ABTS•+ (IC50: 57.75 μg/mL) scavenging assays, alongside robust electron-donating activity in reducing ability tests. Moreover, the SDOLU showed significant inhibition of key enzymes implicated in metabolic and neurodegenerative disorders, including α-amylase (IC50: 531.44 μg/mL), acetylcholinesterase (IC50: 13.23 μg/mL), and carbonic anhydrase II (IC50: 281.02 μg/mL). Collectively, these results highlight the SDOLU as a valuable natural source of multifunctional bioactivities with potential applications in combating oxidative stress and enzyme-related global diseases. Further studies are warranted to validate its therapeutic efficacy and expand its industrial utilization.