Akademik Veri Yönetim Sistemi
JOURNAL OF THERMAL BIOLOGY, cilt.108, 2022 (SCI-Expanded)
Heat stress (HS)-induced intestinal epithelial cell apoptosis may play a pivotal role in intestinal barrier dysfunction in animals. However, the underlying molecular mechanism by which HS induces apoptosis in intestinal epithelial cells is still poorly understood. Herein, a eukaryotic expression vector for an HSP70 gene was constructed and transfected into intestinal porcine epithelial cells (IPEC-J2). Afterward, functional proteomics approaches followed by liquid-chromatography-tandem mass spectrometry (LC-MS/MS) were used to identify interacting proteins. Analysis of HSP70 transfected IPEC-J2 cells revealed 246 differentially expressed proteins (DEPs), and functional annotation indicated that most DEPs were primarily related to ECM-receptor interaction, focal adhesion, and apoptosis. Furtherly, the apoptosis rate and expression levels of apoptosis-related proteins in HSP70 transfected IPEC-J2 cells were detected, we found that the expression of caspase-3, PARP, and Bax were increased, but Bcl-2 were decreased in transfected cells. Lastly, an in vitro and in vivo heat stress model were established to explore the role of HSP70 in intestinal epithelia cell apoptosis. The results of in vitrol study showed that HS-induced cellular apoptosis and increases of caspase-3, PARP, and Bax, but decreased of Bcl-2 in IPEC-J2 cells. In vivo study, the cell apoptosis were induced significantly in the duodenum, cecum, and colon of heat stressed pigs, and upregulation of HSP70 was also detected in colon tissues. Therefore, it has been shown that HSP70 plays a crucial role in heat stress-induced apoptosis and may provide new insights into the molecular mechanisms of epithelial cell apoptosis induced by heat stress in pigs.