Akademik Veri Yönetim Sistemi
FUNCTIONAL AND INTEGRATIVE GENOMICS, cilt.24, sa.10, ss.1-12, 2024 (SCI-Expanded)
Elevated temperatures during grain filling stage, exceeding the optimal range by 3–4 °C, not only results in a substantial
yield reduction in wheat by 10–50% but activates disease and insect infestation. In this research, we introduced heat-tolerant MYB36 and APX-1 gene cassettes into wheat, employing an efficient Agrobacterium mediated transformation protocol,
demonstrating higher transformation efficiency. The study encompassed the assembly of MYB36 and APX-1 gene cassettes,
and confirmation of gene products in Agrobacterium, followed by the transformation of the MYB36 and APX-1 genes into
wheat explants. We were able to select transgenic plant with various combinations. The transgenic plants with APX-1 gene
alone produced medium sized grain and spike whereas with both APX-1 and MYB36 genes expressed individually under
SPS and rd29a promoter respectively showed good tolerance to heat at 32o
C at grain filling/milking stage and produced
relatively bold grains. While non-transgenic plants grains were wrinkled with thin spike showing susceptibility to heat.
This research contributes to the broader scientific understanding of plant stress responses and the combined effectiveness
of MYB36 and APX-1 genes in crop improvement without disturbing normal nutritional values. The gene integration
can serve as a valuable tool in breeding programs aimed at developing heat-tolerant wheat varieties. These findings also
advance our comprehension of the functions of heat-induced genes and lay the foundation for selecting optimal candidates
for in-depth functional studies of heat-responsive MYB36 and APX-1 genes in wheat.