Magnetic molecularly imprinted specific solid-phase extraction for determination of dihydroquercetin from Larix griffithiana using HPLC

Ma X., Zhang X., Lin H., AM A. E., Rabah T., Liu X., ...Daha Fazla

Journal of Separation Science, cilt.43, sa.12, ss.2301-2310, 2020 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 43 Sayı: 12
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1002/jssc.201901086
  • Dergi Adı: Journal of Separation Science
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Aerospace Database, BIOSIS, CAB Abstracts, Chimica, Communication Abstracts, Compendex, EMBASE, Food Science & Technology Abstracts, INSPEC, MEDLINE, Metadex, Veterinary Science Database, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.2301-2310
  • Anahtar Kelimeler: dihydroquercetin, Larix griffithiana, magnetic separation, molecularly imprinted polymers, Tibetan medicine, SELECTIVE EXTRACTION, POLYMER, MICROSPHERES, CHROMATOGRAPHY, RECOGNITION, SEPARATION, SAMPLES, SILICA, ACID
  • Atatürk Üniversitesi Adresli: Evet

Özet

© 2020 WILEY-VCH Verlag GmbH & Co. KGaA, WeinheimThe naturally occurring quercetin flavonoid, dihydroquercetin, is widely distributed in plant tissues and has a variety of biological activities. Herein, a magnetic molecularly imprinted solid-phase extraction was tailor made for selective determination of dihydroquercetin in Larix griffithiana using high-performance liquid chromatography. Amino-functionalized core-shell magnetic nanoparticles were prepared and characterized using scanning electron microscopy, transmission electron microscopy, vibrating sample magnetometry, and infrared spectroscopy. The polymer had an average diameter of 250 ± 2.56 nm and exhibited good stability and adsorption for template molecule, which is enriched by hydrogen bonding interaction. Multiple factors for extraction, including loading, washing, elution solvents, and extraction time, were optimized. The limit of detection was 1.23 μg/g. The precision determined at various concentration of dihydroquercetin was less than 4% and the mean recovery was between 74.64 and 101.80%. It has therefore been shown that this protocol can be used as an alternative extraction to quantify dihydroquercetin in L. griffithiana and purify quercetin flavonoid from other complex matrices.