Akademik Veri Yönetim Sistemi
Annals of Agricultural and Environmental Medicine, cilt.32, sa.4, ss.504-510, 2025 (Scopus)
Introduction and Objective. This study investigated the
antibiotic resistance of bacterial isolates obtained from the gut microbiota of
certain insects (Coleoptera: Hydrophilidae and Helophoridae), which were
collected from aquatic areas in Erzurum Province, Türkiye. This area is
characterised by a low level of human impact, thereby providing a unique
opportunity to investigate the baseline microbial diversity and ecological
roles within relatively pristine aquatic environments. Materials and Method.
The antimicrobial susceptibility of the isolates was assessed using disc
diffusion and minimum inhibitory concentration (MIC) methods. The analysis
encompassed 30 Gram-negative bacteria belonging to the genera Aeromonas,
Acinetobacter, Vibrio, Pseudomonas, Escherichia and Yersinia. Results. The
results indicated that the most resistant bacteria were Aeromonas, Pseudomonas
and Acinetobacter, while enteric bacteria demonstrated greater sensitivity. It
is noteworthy that nitrofurantoin, a commonly used antibiotic for treating
urinary tract infections, exhibited the highest level of resistance among the
antibiotics tested by disc diffusion, followed by cephalosporins and
penicillins. Conclusion. The MIC testing with DKGM and NF kits demonstrated
high resistance to cephalosporins, sulfonamides, polymyxins and monobactams.
Furthermore, two multidrug-resistant (MDR) isolates exhibited resistance to at
least two antibiotic classes. These findings underscore the necessity for
expanded antimicrobial resistance surveillance beyond clinical settings,
extending into environmental samples, and contributing to ongoing research on
resistance mechanisms