Akademik Veri Yönetim Sistemi
Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi, cilt.37, sa.2, ss.139-145, 2023 (Hakemli Dergi)
Malathion (MLT) is widely used as an insecticide in agricultural, veterinary, medicinal, and public health applications. MLT is taken up by the body through the skin, respiratory, and gastrointestinal systems. Rutin (RUT) is a powerful, naturally occurring antioxidant molecule isolated from citrus fruits with low toxicity and powerful anti-oxidant capabilities. The aim of this study was to investigate the protective effects of RUT against MLT-induced gastric toxicity. For this purpose, rats were randomly divided into 5 groups, with 7 animals in each group. Rats were administered orally 100 mg/kg MLT and 50 mg/kg and 100 mg/kg RUT for 28 days. According to the results obtained, while MLT caused lipid peroxidation by increasing malondialdehyde (MDA) levels, it also suppressed antioxidant capacity by decreasing superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) enzyme activities and lowering glutathione (GSH) levels. RUT application decreased the severity of lipid peroxidation and tried to increase antioxidant capacity. In addition, in rats administered MLT, it was determined that inflammation was caused by the increase in nuclear factor kappa B (NF-κB), tumor necrosis factor-alpha (TNFα), cyclooxygenase-2 (COX-2), and inducible nitric oxide synthase (iNOS) mRNA transcript levels, and that the severity of inflammation decreased in a dose-dependent manner with RUT application. When apoptosis, another measured panel, was evaluated, it was determined that the Bax level increased while Bcl-2 decreased as a result of toxicity induced by MLT. However, with the RUT application, it was determined that these parameters had the opposite effect and approached the control group values. When all the results are evaluated, it can be said that RUT application has protective effects on MLT-induced gastric toxicity.