Akademik Veri Yönetim Sistemi
6th International Conference on Advances in Natural and Applied Sciences, Ağrı, Türkiye, 11 - 13 Ekim 2022
Nanoparticles (NPs) are
tiny materials ranging in size from 1 to 100 nm and have unique magnetic,
electrical, optical characteristics differing from the bulk materials. They
have broad spectrum of applications in pharmaceuticals, electronics, optics,
aviation, construction, and automotive industries (Ali, 2020). Several physical and chemical techniques such as
electrochemical, thermal decomposition, chemical vapor deposition, sonochemical
reduction and microwave irradiation have been applied to produce metal NPs
(Cele, 2020). Alternatively, green synthesis of offers an
environmentally-friendly and simple mean for NP preparation. In the present
study, silver NPs were produced by Pseudomonas
aeruginosa OG1 strain. Characterization of NPs were performed by TEM and
XRD. These NPs were used against pathogenic Proteus
mirabilis, which occurs widely in soil and water and shows high level
urease activity and forms clear biofilms. It is the cause of 90% of all Proteus infections and frequently
related with the catheter-associated urinary tract infections (Armbruster et
al., 2018). Silver NPs were applied to inhibit the growth, urease production,
and biofilm formation of P. mirabilis.
Growth inhibition zones of 9 mm and 11 mm were obtained with 100 µg/mL and 200
µg/mL NPs, respectively. 60 % and 85% antibiofilm effects were obtained by 100
µg/mL and 200 µg/mL NPs, respectively. Urease activity of P. mirabilis was completely inhibited in both concentrations. These
results show that the silver NPs can be used as effective antimicrobial,
antibiofilm, and antiurease agents in the fight against pathogens.