Screening of non-alkaloid acetylcholinesterase and carbonic anhydrase isoenzymes inhibitors of Leiotulus dasyanthus (K. Koch) Pimenov & Ostr. (Apiaceae)

KARAKAYA S., Bingol Z., KOCA M., Demirci B., GÜLÇİN İ., Baser K. H. C.

Journal of Essential Oil Research, cilt.32, sa.3, ss.227-241, 2020 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 32 Sayı: 3
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1080/10412905.2020.1746415
  • Dergi Adı: Journal of Essential Oil Research
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Business Source Elite, Business Source Premier, CAB Abstracts, Compendex, Food Science & Technology Abstracts, Veterinary Science Database
  • Sayfa Sayıları: ss.227-241
  • Anahtar Kelimeler: Anticholinesterase, antioxidant, carbonic anhydrase, leiotulus, (TM)-3-carene, pinene, ESSENTIAL OIL, IN-VITRO, BIOLOGICAL EVALUATION, CRYSTAL-STRUCTURE, ANTIMICROBIAL ACTIVITIES, ANTIOXIDANT ACTIVITIES, CHEMICAL-COMPOSITION, ALPHA-PINENE, CHOLINESTERASE, BUTYRYLCHOLINESTERASE
  • Atatürk Üniversitesi Adresli: Evet

Özet

The oxidant, cholinesterase, and carbonic anhydrase inhibitions of essential oils and their major compounds of Leiotulus dasyanthus and molecular docking studies of alpha-pinene and (TM)-3-carene were evaluated in this study. Phenolic constituents and antioxidant activity of samples were determined by Folin-Ciocalteu's, DPPH, and TBA processes. Cholinesterase inhibition effect was evaluated through Ellman's method. GC-FID and GC/MS were used for analysing of essential oils. The root oil got the highest total phenolic content (898.87 mg/g). The utmost activity was shown flower oil (29.31 mu g/mL) in the DPPH test. The root oil indicated considerable inhibition towards acetylcholinesterase (21.92%) and butyrylcholinesterase (91.33%) enzymes, respectively. alpha-pinene and delta-3-carene indicated high acetylcholinesterase inhibitory activity (66.44 and 48.71%, respectively). Cytosolic hCA I and II isoenzymes were influentially inhibited by flower and aerial part oils with 1.694 and 1.677 mu M IC50 values, respectively. 1-Dodecanol (7.8%); beta-pinene (63.3%), alpha-pinene (20.5%); octyl acetate (14.7%); hexyl octanoate (55.1%) were characterized as principle compounds of aerial parts, roots, flowers, and fruits respectively.