Genomic organization and transcription of superoxide dismutase genes (sod1, sod2, and sod3b) and response to diazinon toxicity in platyfish (Xiphophorus maculatus) by using SOD enzyme activity

Bayır M., Özdemir E.

ANIMAL BIOTECHNOLOGY, cilt.34, sa.8, ss.3578-3588, 2023 (SCI-Expanded)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 34 Sayı: 8
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1080/10495398.2023.2178931
  • Dergi Adı: ANIMAL BIOTECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Compendex, EMBASE, Environment Index, Food Science & Technology Abstracts, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.3578-3588
  • Atatürk Üniversitesi Adresli: Evet

Özet

The aim of this study is to determine the effects of 50% of 96h LC50(5.25ppm) diazinon onthe expression of superoxide dismutase (SOD) enzyme genes (sod1,sod2, andsod3b) andSOD enzyme activity at the end of 24, 48, 72, and 96 h in platyfish liver and gill tissues. Tothis end, we determined the tissue-specific distribution ofsod1,sod2, andsod3bgenes andperformedin silicoanalyses in platyfish (Xiphophorus maculatus). It was determined thatmalondialdehyde (MDA) level and SOD enzyme activity were increased in the liver [(43.90EU mg protein1(control), 62.45 EU mg protein1(24 h), 73.17 EU mg protein1(48 h),82.18 EU mg protein1(72h), 92.93 EU mg protein1(96 h)] and gill [(16.44 EU mgprotein1(control), 33.47 EU mg protein1(24h), 50.38 EU mg protein1(48h), 64.62 EUmg protein1(72h), 74.04 EU mg protein1(96h)] tissues of platyfish exposed to diazinon,while the expression of thesodgenes was down-regulated. The tissue-specific distributionof thesodgenes varied, with the tissues and thesodgenes expression were being predom-inant in the liver (628.32 insod1, 637.59 insod2, 888.5 insod3b). Thus, the liver was consid-ered a suitable tissue for further gene expression studies. Based on the phylogeneticanalyses, platyfishsodgenes can be reported to be orthologs ofsod/SODgenes from othervertebrates. Identity/similarity analyses supported this determination. Conserved gene syn-teny proved that there are conservedsodgenes in platyfish, zebrafish, and humans