Akademik Veri Yönetim Sistemi
JOURNAL OF SOIL SCIENCE AND PLANT NUTRITION, cilt.23, sa.4, ss.5683-5694, 2023 (SCI-Expanded)
Purpose Enzymes play an active role in the germination, development, and degradation of seeds. Beta (ss)-mannanase is one of the main enzymes responsible for the hydrolysis of mannans during germination of tomato seeds through hydrolytic degradation of cell walls and weakening of endosperm structures surrounding the embryo and ensuring root formation. Therefore, this study aimed to it was aimed to shorten the germination time and increase the germination rate of tomato seeds by applying immobilized mannanase enzyme to zinc oxide nanoparticle (ZnO Np) purified from Bacillus invictae. Methods The mannanase enzyme was purified from Bacillus invictae, its biochemical properties were determined, and it was immobilized on ZnO Np. Characterization of immobilized mannanase was performed by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopes (SEM), and transmission electron microscope (TEM) analyses. The effects of the immobilized enzyme on the germination of tomato (Solanum lycopersicum) seeds were investigated. The germination index, germination rates, and root and shoot lengths of tomato seeds were determined. Results Mannanase enzyme was partially purified with 3.71% recovery and specific activity of 11.0 (EU mg- 1 protein). Optimum pH and temperature values of partially purified mannanase were determined as pH 6.0 and 40 degrees C, respectively. Immobilized mannanase positively affected both the germination rate and germination time of tomato seeds. In addition, it caused a significant increase in the root and shoot lengths of the seedlings. Conclusions It was concluded that mannanase enzyme immobilized to ZnO Np positively affected seed germination and seedling growth of tomatoes.