Evaluation of Self-Incompatibility of Some Wild Grown Almond Genotypes in Turkey

ÖZKAN G., SAĞBAŞ H. İ., Bozhuyuk M. R., Tuzlaci H. I., Demir A. Y., Binici B., ...Daha Fazla

Erwerbs-Obstbau, cilt.65, sa.1, ss.181-186, 2023 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 65 Sayı: 1
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1007/s10341-022-00789-1
  • Dergi Adı: Erwerbs-Obstbau
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Agricultural & Environmental Science Database, CAB Abstracts, Environment Index, Food Science & Technology Abstracts
  • Sayfa Sayıları: ss.181-186
  • Anahtar Kelimeler: Almond, Allelic diversity, Self-incompatibility, Self-compatibility, Germplasm, S-ALLELES, GENETIC DIVERSITY, IDENTIFICATION, PRIMERS
  • Atatürk Üniversitesi Adresli: Evet

Özet

© 2022, The Author(s), under exclusive licence to Der/die Autor(en), exklusiv lizenziert an Springer-Verlag GmbH Deutschland, ein Teil von Springer Nature.Almond in general shows self-incompatibility (SI) controlled by the S‑allele. Self-compatible (SC or Sf) cultivars have an advantage in breeding programs because SC traits can be transferred to new individuals. In new orchard designs, SC cultivars give higher yield per unit area because problems with fertilization in general are eliminated. Thus, determination of almond germplasm polymerase chain reaction (PCR)-based identification of SC- and SI-alleles is useful for selecting suitable pollinizers and the design of crossing in almond breeding programs. In this study, a total of 40 wild grown almond genotypes from Elazig province of Turkey were evaluated for SI and SC characteristics by PCR assay. In PCR analysis, among used consensus primers, AS1IIF/AmyC5R showed a high number of amplifications of seven SI-alleles (S1, S2, S5, S7, S10, S11 and S13) and Sf-allele. The almond genotypes frequently had S2-allele (eight genotypes each), followed by S1, S7 and S13 (seven genotypes each) and S11 (five genotypes) compared to the other S‑alleles. The results showed that detection of S‑alleles by RNase activity and PCR analysis by consensus primers clearly indicated right parent selection, which is important to increase almond yield.