Electrically controlled mRNA delivery using a polypyrrole-graphene oxide hybrid film to promote osteogenic differentiation of human mesenchymal stem cells

Kim H., Solak K., Han Y., Cho Y., Koo K., Kim C., ...Daha Fazla

NANO RESEARCH, cilt.15, sa.10, ss.9253-9263, 2022 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 15 Sayı: 10
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1007/s12274-022-4613-y
  • Dergi Adı: NANO RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Biotechnology Research Abstracts, Compendex, INSPEC, Metadex
  • Sayfa Sayıları: ss.9253-9263
  • Anahtar Kelimeler: messenger ribonucleic acid (mRNA) delivery, polypyrrole, graphene oxide, electrical release, mesenchymal stem cells, DRUG-DELIVERY, GENE, RELEASE, FLUORESCENCE, COMPOSITE, DNA, NANOPARTICLES, EXPRESSION, DIVERSITY, THERAPY
  • Atatürk Üniversitesi Adresli: Evet

Özet

Direct messenger ribonucleic acid (mRNA) delivery to target cells or tissues has revolutionized the field of biotechnology. However, the applicability of regenerative medicine is limited by the technical difficulties of various mRNA-loaded nanocarriers. Herein, we report a new conductive hybrid film that could guide osteogenic differentiation of human adipose-derived mesenchymal stem cells (hADMSCs) via electrically controlled mRNA delivery. To find optimal electrical conductivity and mRNA-loading capacity, the polypyrrole-graphene oxide (PPy-GO) hybrid film was electropolymerized on indium tin oxide substrates. We found that the fluorescein sodium salt, a molecule partially mimicking the physical and chemical properties of mRNAs, can be effectively absorbed and released by electrical stimulation (ES). The hADMSCs cultivated on the PPy-GO hybrid film loaded with pre-osteogenic mRNAs showed the highest osteogenic differentiation under electrical stimulation. This platform can load various types of RNAs thus highly promising as a new nucleic acid delivery tool for the development of stem cell-based therapeutics.