Isolation of 3 Flavonoids from Mentha longifolia (L.) Hudson subsp longifolia and Determination of Their Genotoxic Potentials by Using the E. coli WP2 Test System

BARIŞ Ö., KARADAYI M., YANMIS D., GÜVENALP Z., Bal T., GÜLLÜCE M.

JOURNAL OF FOOD SCIENCE, cilt.76, sa.9, 2011 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 76 Sayı: 9
  • Basım Tarihi: 2011
  • Doi Numarası: 10.1111/j.1750-3841.2011.02405.x
  • Dergi Adı: JOURNAL OF FOOD SCIENCE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Anahtar Kelimeler: antimutagenicity, apigenin, E, coli WP2 test, Mentha longifolia, mutagenicity, REVERSE MUTATION ASSAY, ANTIMUTAGENIC ACTIVITY, MUTAGENICITY ASSAY, PHENOLIC-COMPOUNDS, SALMONELLA ASSAY, ESSENTIAL OILS, AMES TEST, EXTRACT, ANTIOXIDANT, APIGENIN
  • Atatürk Üniversitesi Adresli: Evet

Özet

Flavonoids, abundant in most of plant species, are widely used in medicine and development studies on phytotherapeutic drugs due to their various biological activities. In the present study, 3 flavonoids, apigenin-7-O-glucoside, apigenin-7-O-rutinoside, and apigenin-7-O-glucuronide, were isolated from Mentha longifolia (L.) Hudson subsp. longifolia by using E. coli WP2 genotoxicity assay guided fractionation procedures. Later, the mutagenic and antimutagenic properties of each flavonoid were evaluated by using the same genotoxicity assay. The results showed that all the test compounds have significant antimutagenic activity at tested concentrations with or without S9 activation. The inhibition rates were between 25.3% (apigenin-7-O-glucoside with S92.0 mu M/plate) and 59.0% (apigenin-7-O-rutinoside without S92.0 mu M/plate). In conclusion, the results revealed that the 3 flavonoids from Mentha longifolia (L.) Hudson subsp. longifolia have significant antimutagenic activity, and the findings of the present study are valuable for further investigations, focus on the phytotherapeutic drug discovery.