Inhibition effects of some pesticides and heavy metals on carbonic anhydrase enzyme activity purified from horse mackerel (Trachurus trachurus) gill tissues

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Caglayan C., Taslimi P., Türk C., Gülçin İ., Kandemir F. M., Demir Y., ...Daha Fazla

Environmental Science and Pollution Research, cilt.27, sa.10, ss.10607-10616, 2020 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 27 Sayı: 10
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1007/s11356-020-07611-z
  • Dergi Adı: Environmental Science and Pollution Research
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, IBZ Online, ABI/INFORM, Aerospace Database, Agricultural & Environmental Science Database, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, EMBASE, Environment Index, Geobase, MEDLINE, Pollution Abstracts, Veterinary Science Database, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.10607-10616
  • Anahtar Kelimeler: Carbonic anhydrase, Horse mackerel, Trachurus trachurus, Gill, Heavy metal, Pesticides, BIOLOGICAL EVALUATION, PURIFICATION, ACETYLCHOLINESTERASE, LIVER, WATER, BUTYRYLCHOLINESTERASE, POTENT, COPPER, FISH, BROMOPHENOLS
  • Atatürk Üniversitesi Adresli: Evet

Özet

The gill tissue is the main site of metabolic enzymes or compensation, with the kidney tissue playing a supporting role. At the gill tissue, carbonic anhydrase enzymes (CAs) catalyze the hydration of CO2 to HCO3- and H+ for production to the H2O. In this work, the CA enzyme was purified from horse mackerel (Trachurus trachurus) gill with a specific activity of 21,381.42 EU/mg, purification fold of 150.61, total activity of 2347.68 EU/mL, and a yield of 16.13% using sepharose 4B-L-tyrosine-sulfanilamide affinity gel chromatography. For recording the enzyme purity, gel electrophoresis was performed, and single band was seen. The molecular weight of this enzyme was found approximately 35 kDa. Also, the inhibitory effects of different pesticides such as thiram, clofentezine, propineb, deltamethrin, azoxystrobin, and thiophanate and heavy metal ions such as Fe2+, Cu2+, Co2+, Pb2+ Hg2+, and As3+ on horse mackerel gill tissue CA enzyme activities were investigated. Our results indicated that these pesticides and metal ions showed inhibitory effects at low nanomolar and millimolar concentrations for fish gill CA enzymes, respectively.