Akademik Veri Yönetim Sistemi
Türk Hijyen ve Deneysel Biyoloji Dergisi, cilt.80, sa.2, ss.135-144, 2023 (Scopus)
Objective: The rapid spread of the coronavirus disease, which started in 2019, has caused it to become a global epidemic. To control the spread of the coronavirus epidemic, some prevention procedures such as wearing face masks, maintaining social distance and hand hygiene have begun to be implemented. Accordingly, the use of disinfectants in public places such as transportation and shopping has gained importance. However, studies have shown that long-term use of disinfectants with different pH values triggers the development of itching, redness, urticaria and even allergic rhinitis. The low saturated fatty acids and high amounts of olive oil found in Moringa oleifera (MO) leaves, flowers, green beans, seeds and seed oil are excellent sources of nutrients and vitamins. Today, MO is used in a variety of skin care applications. In addition, the leaf of MO also has antiretroviral, antimicrobial, antioxidant, antifungal properties. The aim of this study is to prevent toxicity against different pH changes by using MO plant. Methods: In our study, a human fibroblast cell line was grown under the conditions specified by the manufacturer and inoculated into 96 well plates and a culture medium with different pH (5.0, 6.0, 7.0 and 8.0) was prepared and different doses of MO (20, 40, 80 and 160 μg/ml) were prepared. It is planned to eliminate the toxicity caused by using it for 24 hours. At the end of the study, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), Glutathione Reductase (GR), and Lactate Dehydrogenase (LDH) tests were used. Results: According to the results of our study, cell viability increased from %69 to %89 compared to positive control at pH 5.0. The vitality that decreased to %80 at pH 6.0 increased to 143 as a result of the application of the highest concentration of MO (160 μg/ml). While it increased from %79 to %115 at pH 7.0, the damage received by fibroblast cells at pH 8.0 was determined to be high (viability rate %66), and it was observed that this toxicity was removed, and the vitality was increased up to %93. All results were statistically evaluated and found to be significant (P<0.05 and P<0.01). Conclusion: Considering the results obtained, MO is recommended to be used at doses of 80 and 160 μg/ml, as it has a protective effect. The LDH and GR results show a correlation with MTT.