Determination of olanzapine for therapeutic drug monitoring in schizophrenia patients by LC/MS method

ALBAYRAK M., KADIOĞLU Y., YAMAN M. E., ŞENOL O., Oral E.

BIOMEDICAL CHROMATOGRAPHY, cilt.33, sa.4, 2019 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 33 Sayı: 4
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1002/bmc.4468
  • Dergi Adı: BIOMEDICAL CHROMATOGRAPHY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Anahtar Kelimeler: LC-MS method, olanzapine, schizophrenia, validation, PERFORMANCE-LIQUID-CHROMATOGRAPHY, TANDEM MASS-SPECTROMETRY, HUMAN PLASMA, PSYCHOTROPIC-DRUGS, INTRAMUSCULAR OLANZAPINE, ANTIPSYCHOTIC-DRUGS, DOUBLE-BLIND, HUMAN BLOOD, VALIDATION, SERUM
  • Atatürk Üniversitesi Adresli: Evet

Özet

Olanzapine is an atypical antipsychotic drug from the thienobenzodiazepine family which displays efficacy in patients with schizophrenia and related psychoses. A novel LC/MS method was developed and validated for determination of olanzapine in schizophrenia patients' plasma. A liquid-liquid extraction procedure was carried out using 5 mL diethyl ether-diisopropyl ether mixture (1:1, v/v). Average recovery of the extraction procedure was 94.8%. Chromatographic separation was performed on reversed-phase C-18 column (250 x 2.0 mm, 5 mu m) using mixture of deionized water (trifluoro acetic acid 0.1%)-acetonitrile (20:80, v/v) as mobile phase at a flow rate of 1 mL/min. Irbesartan was used as internal standart and total run time was 2.5 min. Mass spectrometric analysis were carried out in selective-ion montoring mode, and detected olanzapine at m/z 313.1 and IS at m/z 429.4 in all forms of the ions. The calibration curve of olanzapine was linear in the range 2-300 ng/mL (r(2) > 0.9993). The interday and intraday precisions (RSD) were <7.55%, and accuracy was >7.59% (n = 6). The proposed study was successfully validated with respect to the US Food and Drug Administration guidelines.