Mitigation of salinity stress in cucumber seedlings by exogenous hydrogen sulfide

TURAN M., EKİNCİ M., KUL R., Boynueyri F. G., YILDIRIM E.

JOURNAL OF PLANT RESEARCH, cilt.135, sa.3, ss.517-529, 2022 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 135 Sayı: 3
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1007/s10265-022-01391-y
  • Dergi Adı: JOURNAL OF PLANT RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Agricultural & Environmental Science Database, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, EMBASE, MEDLINE, Pollution Abstracts, Veterinary Science Database
  • Sayfa Sayıları: ss.517-529
  • Anahtar Kelimeler: Cucumis sativus L, Hydrogen sulfide, Physiology, Plant growth, Salinity, SALT STRESS, NITRIC-OXIDE, STRAWBERRY PLANTS, OXIDATIVE STRESS, PROTECTIVE ROLE, SALICYLIC-ACID, TOLERANCE, METABOLISM, PROLINE, DONOR
  • Atatürk Üniversitesi Adresli: Evet

Özet

This research hypothesized that tolerance of cucumber seedlings to salinity stress could be increased by hydrogen sulfide (H2S) treatments. In pot experiments, the cucumber seedlings were exposed to three levels of salt stress (0, 50 and 100 mM NaCl), and NaHS as H2S donor was foliar applied to the cucumber seedlings at five different doses (0, 25, 50, 75 and 100 mu M). The effects of the treatments on cucumber seedlings were tested with plant growth properties as well as physiological and biochemical analyses. As the salinity level increased, plant growth properties and chlorophyll reading value (SPAD) decreased. However, H2S treatments significantly mitigated the impact of salinity. Salt stress elevated the membrane permeability (MP) and decreased the leaf relative water content (LRWC). H2S applied leaves had lower MP and higher LRWC than non-H2S applied leaves. On the other hand, photosynthetic properties (net photosynthetic rate, stomatal conductance, transpiration rate and intercellular CO2 concentration) of the seedlings under salt stress conditions were decreased but this decrease was considerably relieved by H2S treatment. The K/Na and Ca/Na ratios under salt stress conditions were higher in H2S-applied plants than in non-applied plants. Furthermore, antioxidant enzyme activity [(catalase (CAT), peroxidase (POD) and superoxide dismutase (SOD)] and hydrogen peroxide (H2O2), malondialdehyde (MDA), proline, and sucrose concentration in the leaves increased with salinity stress whereas they were reduced with H2S treatments under salt stress. Mitigation of salt stress damage in cucumber using H2S treatment can be expounded via modulation of enzyme activity, nutrient content, reactive oxygen species (ROS) formation, and osmolytes accumulation.