Free-standing Graphene/Poly(methylene blue)/AgNPs Composite Paper for Electrochemical Sensing of NADH

Topçu E., Dagcı K., Alanyalıoğlu M.

Electroanalysis, cilt.28, sa.9, ss.2058-2069, 2016 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 28 Sayı: 9
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1002/elan.201600108
  • Dergi Adı: Electroanalysis
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.2058-2069
  • Anahtar Kelimeler: graphene composite paper, methylene blue, surface-confined electropolymerization, NADH, amperometric sensor, REDUCED GRAPHENE OXIDE, NICOTINAMIDE ADENINE-DINUCLEOTIDE, METHYLENE-BLUE, CARBON NANOTUBES, SENSITIVE NADH, FILMS, SHEETS, NANOPARTICLES, FABRICATION, ADSORPTION
  • Atatürk Üniversitesi Adresli: Evet

Özet

Highly flexible graphene/poly(methylene blue)/AgNPs composite paper was successfully prepared for amperometric biosensing of NADH. For this purpose, a dispersion including graphene oxide (GO), methylene blue (MB) and silver nanoparticles (AgNPs) was prepared and GO/MB/AgNPs paper was acquired by vacuum-filtration of this dispersion through a suitable membrane. After peeling it off from membrane, it was transformed to rGO/MB/AgNPs paper by performing reduction with hydriodic acid. In a three-electrode cell, which is containing 0.1M phosphate buffer solution (pH: 9.0), rGO/MB/AgNPs paper was used as working electrode and rGO/poly(MB)/AgNPs composite paper was generated by surface-confined electropolymerization of MB using successive cyclic voltammetry approach in a suitable potential window. Characterization of this composite paper was carried out by using scanning electron microscopy, scanning tunneling microscopy, X-ray photoelectron spectroscopy, powder X-ray diffraction spectroscopy, Raman spectroscopy, four-point probe conductivity measurement and cyclic voltammetry techniques. Flexible rGO/poly(MB)/AgNPs composite paper has demonstrated high sensitivity, wide linear range and low detection limit for amperometric quantification of NADH.