Metabolite analysis in <i>Curcuma domestica</i> using various GC-MS and LC-MS separation and detection techniques

Herebian D., Choi J., HASSIBELNABY A. M. A., Shim J., Spiteller M.

BIOMEDICAL CHROMATOGRAPHY, cilt.23, sa.9, ss.951-965, 2009 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 23 Sayı: 9
  • Basım Tarihi: 2009
  • Doi Numarası: 10.1002/bmc.1207
  • Dergi Adı: BIOMEDICAL CHROMATOGRAPHY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.951-965
  • Atatürk Üniversitesi Adresli: Hayır

Özet

The metabolic profile of polar (methanol) and non-polar (hexane) extracts of Curcuma domestica, a widely used medicinal plant, was established using various different analytical techniques, including GC-FID, GC-MS, HR-GC-MS and analytical HPLC-ESI-MS/MS by means of LTQ-Orbitrap technology. The major non-volatile curcuminoids curcumin, demethoxycurcumin and bisdemethoxycurcumin were identified when their chromatographic and precursor ion masses were compared with those of authentic standard compounds. In this paper we describe for the first time a GC/MS-based method for metabolic profiling of the hydrophilic extract. We also identified 61 polar metabolites as TMS derivatives. Copyright (C) 2009 John Wiley & Sons, Ltd.