Anticholinergic and antioxidant activities of usnic acid-an activity-structure insight.

Cetin C., Gülçin İ.

Toxicology reports, cilt.6, ss.1273-1280, 2019 (ESCI) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 6
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1016/j.toxrep.2019.11.003
  • Dergi Adı: Toxicology reports
  • Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI), Scopus
  • Sayfa Sayıları: ss.1273-1280
  • Anahtar Kelimeler: Usnic acid, Antioxidant activity, Acetylcholinesterase, Butyrylcholinesterase, Enzyme inhibition, IN-VITRO ANTIOXIDANT, POTENT CARBONIC-ANHYDRASE, POLYPHENOL CONTENTS, ANTIRADICAL ACTIVITIES, INHIBITORY PROPERTIES, CRYSTAL-STRUCTURE, CAFFEIC ACID, ACETYLCHOLINESTERASE, DERIVATIVES, BUTYRYLCHOLINESTERASE
  • Atatürk Üniversitesi Adresli: Evet

Özet

Usnic acid, as active dibenzofuran derivative, isolated and characterized from some lichen species. The aim of manuscript was to evaluate antioxidant, anticholinergic and antidiabetic potentials of usnic acid as an important natural product. Antioxidant profile of usnic acid determined by eight distinguishes bioanalytical antioxidant methods including 1,1-diphenyl-2-picrylhydrazyl (DPPH center dot), 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS(center dot+)), superoxide anion radical (O-2(center dot-)) and N,N-dimethyl-p-phenylenediamine (DMPD center dot+) scavenging activities, cupric ion (Cu2+), ferric ion (Fe3+) and Fe3+-TPTZ reducing abilities and ferrous ion (Fe3+) chelating activity. Usnic acid was found as potent DPPH center dot (IC50: 49.50 mu g/mL), DMPD center dot+ (IC50: 33.00 mu g/mL), O-2(center dot-) (IC50: 18.68 mu g/mL), and ABTS(center dot+) (IC50: 10.41 mu g/mL) scavenging effects. Also, the inhibition effects of usnic acid were tested against some metabolic enzymes including acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) linked to neurodegenerative diseases. Both enzymes play important roles in cholinergic transmission by hydrolyzing the neurotransmitter acetylcholine in cholinergic synapses, central nervous system, neuromuscular junctions and autonomic ganglia. Their inhibitors were used for clinical treatment of some neurodegenerative conditions including myasthenia gravis, Alzheimer's disease, apathy, glaucoma, postural tachycardia syndrome and dementia. Furthermore, usnic acid showed the potent inhibition profiles against AChE (IC50: 1.273 nM) and BChE (IC50: 0.239 nM) enzymes. The results clearly showed that usnic acid is an important natural product with antioxidant and anticholinergic potentials.