Purification, kinetic characterization of thermostable multicopper oxidase from the oyster mushroom and its versatility for greener agro-pulp bio bleaching in the paper industry


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Kaur M., Sharma S., Kaur I., Sodhi H. S., Choudhary R., ERCİŞLİ S., ...Daha Fazla

Cellular and Molecular Biology, cilt.70, sa.2, ss.1-9, 2024 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 70 Sayı: 2
  • Basım Tarihi: 2024
  • Doi Numarası: 10.14715/cmb/2024.70.2.1
  • Dergi Adı: Cellular and Molecular Biology
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, CAB Abstracts, EMBASE, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.1-9
  • Anahtar Kelimeler: Biobleaching, Characterization, Enzyme kinetics, Laccase, Pleurotus florida
  • Atatürk Üniversitesi Adresli: Evet

Özet

Production of a thermostable laccase from Pleurotus florida was reported for the first time, both in submerged and solid-state fermentation using agro-industrial residues. This enzyme was purified using ammonium sulphate precipitation (60-90%), Sephadex G-100 and DEAE column ion exchange chromatography, respectively. The laccase was purified to 21.49 fold with an apparent molecular weight of 66 kDa and had an optimal pH of 5 with temperature stability at 60°C. Metal ions such as Cu2+ (91.26 μmol/mL/min), Mg2+ (68.15 μmol/ mL/min), and Fe2+ (1.73 μmole/mL/min) enhanced the laccase activity, but Fe2+ (1.73 μmol/mL/min) inhibited the enzyme activity. The purified laccase had Km and Vmax of 16.68 mM and 26.73 μmole/mL/min for guaiacol as a substrate. The isolated enzyme was characterized by FT-IR which revealed bands at 3655.0/cm, 2894.7/ cm, and 1151.7/cm corresponding to primary amines, C-H stretch, and amide –III, respectively. The enzymatic bio bleaching of paddy straw pulp was found to be most effective which resulted in a lowering of kappa number and yellowness by 19.47% and 17.84% whereas an increase in brightness and whiteness by 41.92% and -19.61%. Thus, this might be stated that the crude laccase from P. florida can be exploited to reduce the toxic waste load for managing environmental pollution and helps in enhancing the yield and quality of the paper.