Bufferized solvent extraction and HPLC fluorometric detection method for sarafloxacin in pig and chicken muscles

Choi J., Na T. W., Mamun M. I. R., HASSIBELNABY A. M. A., Shin E., Park J., ...Daha Fazla

BIOMEDICAL CHROMATOGRAPHY, cilt.25, sa.3, ss.405-411, 2011 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 25 Sayı: 3
  • Basım Tarihi: 2011
  • Doi Numarası: 10.1002/bmc.1463
  • Dergi Adı: BIOMEDICAL CHROMATOGRAPHY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.405-411
  • Atatürk Üniversitesi Adresli: Hayır

Özet

In this study, amethod for the detection of sarafloxacin in pig and chicken muscles was developed using HPLC-FLD as a regulatory residue technique. Good extraction efficiency was achieved using a mixture of 1% orthophosphoric acid-0.2 M MgCl2 in water and acetonitrile as an extraction solvent, and n-hexane partitioning and centrifugation for cleanup was used in the absence of dehydration. Specificity, linearity, detection and quantification limits, recovery, accuracy and precision were all validated, and all results were sufficient for the SARA regulatory residue method in pig and chicken muscles. The method developed and described herein was not only simple but also reliable, and was applied to market samples to determine their residue contents. Copyright (C) 2010 John Wiley & Sons, Ltd.