Benefits of the antioxidant and anti-inflammatory activity of etoricoxib in the prevention of ovarian ischemia/reperfusion injury induced experimentally in rats

YAPÇA Ö. E., TURAN M., YILMAZ İ., SALMAN S., GÜLABOĞLU M., SÜLEYMAN H.

JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH, cilt.40, sa.6, ss.1674-1679, 2014 (SCI-Expanded) identifier identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 40 Sayı: 6
  • Basım Tarihi: 2014
  • Doi Numarası: 10.1111/jog.12373
  • Dergi Adı: JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1674-1679
  • Anahtar Kelimeler: etoricoxib, ischemia, ovary, oxidants, rat, ISCHEMIA-REPERFUSION INJURY, FOCAL CEREBRAL-ISCHEMIA, OXIDATIVE STRESS, ADNEXAL TORSION, CYCLOOXYGENASE-2, EXPRESSION, IMPACT, TISSUE, COX-1, LUNG
  • Atatürk Üniversitesi Adresli: Evet

Özet

Aim This study is a biochemical investigation of the effect of etoricoxib, a selective cyclooxygenase (COX)-2 inhibitor, on ischemia/reperfusion (I/R) injury experimentally induced in rat ovaries. Methods Experimental animals were divided into four groups: (i) ovarian ischemia/reperfusion (IRG); (ii) 30mg/kg etoricoxib+ovarian ischemia/reperfusion (EIRG-30); (iii) 60mg/kg etoricoxib+ovarian ischemia/reperfusion (EIRG-60); and (iv) a sham operation (SG) control group. Results The results showed levels of malondialdehyde in the IRG, EIRG-30, EIRG-60 and SG group ovarian tissue of 20.2 +/- 3.4, 11.2 +/- 3.2, 5.5 +/- 1.9 and 3.8 +/- 1.5mol/g protein, respectively. Myeloperoxidase activity for these groups was 24.2 +/- 6.7, 13 +/- 2.4, 4 +/- 1.8 and 3.5 +/- 1.9U/g protein, and total glutathione levels were 1.6 +/- 0.8, 4.5 +/- 1.9, 6.5 +/- 1.9 and 7.5 +/- 2.4nmol/g protein, respectively. COX-1 activity in IRG, EIRG-30, EIRG-60 and SG group rat ovarian tissue was 5.0 +/- 2.8, 12.2 +/- 2.4, 16.7 +/- 2.8 and 17.5 +/- 4.7U/mg protein, and COX-2 activity was 18.3 +/- 2.7, 3.5 +/- 1, 1.8 +/- 0.7 and 0.7 +/- 0.3U/mg protein, respectively. Conclusion Etoricoxib prevented oxidative damage induced with I/R in rat ovarian tissue. This property of etoricoxib suggests that it can be clinically beneficial in the prevention of damage that may arise with reperfusion by detorsion for the protection of the ovaries against torsion.