Genetic polymorphism in Kappa-casein gene detected by PCR-RFLP in cattle

DOĞRU Ü., ÖZDEMİR M., ERCİŞLİ S.

JOURNAL OF APPLIED ANIMAL RESEARCH, cilt.33, sa.1, ss.65-68, 2008 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 33 Sayı: 1
  • Basım Tarihi: 2008
  • Doi Numarası: 10.1080/09712119.2008.9706898
  • Dergi Adı: JOURNAL OF APPLIED ANIMAL RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.65-68
  • Anahtar Kelimeler: Kappa-casein, PCR-RFLP, genetic polymorphism, VARIANTS
  • Atatürk Üniversitesi Adresli: Evet

Özet

Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method has been used to detect polymorphism in Kappa-casein gene in cattle. A 351 bp Kappa-casein gene fragment was amplified and the PCR products were digested with four different restriction enzymes i.e. Alu I, Hind III, Hinf I and Taq I. All these enzymes were able to distinguish between Kappa-casein A and B types, however Hind III, Hinf I and Taq I were found more effective for kappa-casein variants than Alu I.