Genetic polymorphism in Kappa-casein gene detected by PCR-RFLP in cattle
JOURNAL OF APPLIED ANIMAL RESEARCH, cilt.33, sa.1, ss.65-68, 2008 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 33 Sayı: 1
- Basım Tarihi: 2008
- Doi Numarası: 10.1080/09712119.2008.9706898
- Dergi Adı: JOURNAL OF APPLIED ANIMAL RESEARCH
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.65-68
- Anahtar Kelimeler: Kappa-casein, PCR-RFLP, genetic polymorphism, VARIANTS
- Atatürk Üniversitesi Adresli: Evet
Özet
Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method has been used to detect polymorphism in Kappa-casein gene in cattle. A 351 bp Kappa-casein gene fragment was amplified and the PCR products were digested with four different restriction enzymes i.e. Alu I, Hind III, Hinf I and Taq I. All these enzymes were able to distinguish between Kappa-casein A and B types, however Hind III, Hinf I and Taq I were found more effective for kappa-casein variants than Alu I.