Clinical Investigation of Leukocyte DNA Damage in COVID-19 Patients

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Doğan H., Kara A., Çankaya E., Balkan E., Gürbüz M. A., Kızılkaya Y. M., ...Daha Fazla

CURRENT ISSUES IN MOLECULAR BIOLOGY, cilt.45, sa.2, ss.963-974, 2023 (SCI-Expanded)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 45 Sayı: 2
  • Basım Tarihi: 2023
  • Doi Numarası: 10.3390/cimb45020062
  • Dergi Adı: CURRENT ISSUES IN MOLECULAR BIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, CAB Abstracts, EMBASE, Food Science & Technology Abstracts, Veterinary Science Database, Directory of Open Access Journals
  • Sayfa Sayıları: ss.963-974
  • Atatürk Üniversitesi Adresli: Evet

Özet

Abstract: This prospective cross-sectional study aimed to evaluate leukocyte DNA damage in coronavirus

disease (COVID-19) patients. In this study, 50 COVID-19-positive patients attending the

Erzurum City Hospital Internal Medicine Outpatient Clinic and 42 control group patients were included.

DNA damage was detected in living cells through leukocyte isolation in 50 COVID-19-positive

patients using the comet assay method. DNA tail/head (olive) moments were evaluated and

compared. White blood cells (WBC), red blood cells (RBC), hemoglobin (HGB), neutrophils (NEU),

lymphocytes (LYM), eosinophils (EO), monocytes (MONO), basophils (BASO), platelets (PLT), and

the neutrophil/lymphocyte ratio (NLR) were analyzed. The RBC, lymphocyte, eosinophil, and monocyte

means were significantly higher in the control group (p < 0.05), whereas the HGB and neutrophile

means were significantly higher in the study group (p < 0.05). There were significant negative

correlations between COVID-19 and RBC (r = −0.863), LYM (r = −0.542), EO (r = −0.686), and MONO

(r = −0.385). Meanwhile, there were significant positive correlations between COVID-19 and HGB (r

= 0.863), NEU (r = 0.307), tail moment (r = 0.598), and olive moment (r = 0.582). Both the tail and olive

moment mean differences were significantly higher in the study group, with higher ranges (p <

0.05). COVID-19 infection caused statistically significant increases in both the tail and olive damage

percentage in patients, causing DNA damage. Lastly, the NLR rate was associated with the presence

and progression of COVID-19.