Isolation of Bacillus zhangzhouensis OBB Liu et al. (Caryophanales: Bacillaceae) from native Polyphylla fullo (L., 1758) (Coleoptera: Scarabaeidae) larvae, PCR-based detection of cry1 gene and evaluation of its biological control potential Yerel Polyphylla fullo (L., 1758) (Coleoptera: Scarabaeidae) larvalarından Bacillus zhangzhouensis OBB Liu et al. (Caryophanales: Bacillaceae)'nin izolasyonu, cry1 geninin PCR tabanlı tespiti ve biyolojik kontrol potansiyelinin değerlendirilmesi

BAKIR BOĞA Ö., Başaran Kurbanoğlu E.

Turkiye Entomoloji Dergisi, cilt.49, sa.1, ss.39-51, 2025 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 49 Sayı: 1
  • Basım Tarihi: 2025
  • Doi Numarası: 10.16970/entoted.1613003
  • Dergi Adı: Turkiye Entomoloji Dergisi
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, CAB Abstracts, Veterinary Science Database, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.39-51
  • Anahtar Kelimeler: Bacillus zhangzhouensis OBB, cry genes, pesticides, Polyphylla fullo, SDS-PAGE
  • Atatürk Üniversitesi Adresli: Evet

Özet

Bacillus zhangzhouensis OBB Liu et al. (Caryophanales: Bacillaceae) isolate was obtained from Polyphylla fullo (L., 1758) (Coleoptera: Scarabaeidae) larvae in the Microbiology Laboratory of the Biology Department of the Faculty of Science of Atatürk University in 2024. Additionally, the presence of the cry1 gene was identified. After endospore staining of the isolate, the presence of crystal protein was detected by phase contrast microscopy and SEM analysis. As a result of PCR, only the presence of the cry1 gene was detected and confirmed. The total protein contents were compared with those of Bacillus thuringiensis (Berliner, 1915) (Bacteria: Bacillaceae) by performing SDS-PAGE analysis using a crystal protein and spore mixture. B. zhangzhouensis OBB showed bands ̴ 250 kDa and ̴ 80 kDa, while B. thuringiensis showed bands corresponding to ̴ 70 kDa and ̴ 45 kDa. Probit analysis was used to determine the LC50 value of the isolates, and the Abbott method was used to determine the mortality percentages of the larvae. Spore-crystal mixtures of B. thuringiensis and B. zhangzhouensis OBB isolates were tested against P. fullo larvae at doses of 1000, 2000, and 4000 ppm. The highest mortality rate was determined in the spore-crystal mixture of B. zhangzhouensis OBB isolate at 4000 ppm dose.