Potent Inhibitory Effects of Some Phenolic Acids on Lactoperoxidase

KÖKSAL Z., Alim Z., Beydemir S., ÖZDEMİR H.

JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY, cilt.30, sa.11, ss.533-538, 2016 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 30 Sayı: 11
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1002/jbt.21819
  • Dergi Adı: JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.533-538
  • Atatürk Üniversitesi Adresli: Evet

Özet

Lactoperoxidase (LPO) plays a key role in immune response against pathogens. In this study, we examined the effects of some phenolic acids on LPO. For this purpose, bovine milk LPO was purified 380.85-fold with a specific activity of 26.66EU/mg and overall yield of 73.33% by using Amberlite CG-50 H+ resin and CNBr-activated Sepharose-4B-l-tyrosine-sulfanilamide affinity chromatography. After purification, the in vitro effects of phenolic acids (tannic acid, 3,4-dihydroxybenzoic acid, 3,5- dihydroxybenzoic acid, chlorogenic acid, sinapic acid, 4-hydroxybenzoic acid, vanillic acid, salicylic acid, and 3-hydroxybenzoic acid) were investigated on LPO. These phenolic acids showed potent inhibitory effect on LPO. K-i values for these phenolic acids were found as 0.0129nM, 0.132M, 0.225M, 0.286M, 0.333M, 2.33M, 10.82M, 0.076mM, and 0.405mM, respectively. Sinapic acid and 4-hydroxybenzoic acid exhibited noncompetitive inhibition; 3,4-dihydroxybenzoic acid showed uncompetitive inhibition, and other phenolic acids showed competitive inhibition.