Production of (S)-(-)-1-(1'-Napthyl) Ethanol by Rhodotorula glutinis Isolate Using Ram Horn Peptone

KURBANOĞLU E. B., ZILBEYAZ K., Kurbanoglu N. I., TAŞKIN M., KILIÇ H.

TURKISH JOURNAL OF CHEMISTRY, cilt.32, sa.6, ss.685-692, 2008 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 32 Sayı: 6
  • Basım Tarihi: 2008
  • Dergi Adı: TURKISH JOURNAL OF CHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.685-692
  • Anahtar Kelimeler: Acetonapthone, bioreduction, microbial reduction, asymmetric reduction, ENANTIOSELECTIVE REDUCTION, BAKERS-YEAST, CITRIC-ACID, ACETOPHENONE, BIOREDUCTION, HYDROLYSATE
  • Atatürk Üniversitesi Adresli: Evet

Özet

The bioreduction of 1-acetonaphthone 1 by locally isolated Rhodotorula glutinis strains using ram horn peptone (RHP) gave (S)-(-)-1-(1'-naphthyl) ethanol ( 2), an important pharmaceutical intermediate. R. glutinis strains were isolated from the water of fermented Salix leaves. Optimum fermentation conditions for the production of 2 were 200 rpm, 32 degrees C, and pH 6.5. The production of 2 with excellent enantiomeric excess (> 99%), and good conversion (100%) and yield (78%) under optimum conditions was achieved up to preparative scale via a fermenter. Wide temperature, pH, time, and agitation ranges did not affect the enantiomeric excess (ee) of the bioreduction product. A simple process for the isolation of R. glutinis was also developed.