Gas chromatography-mass spectrometry method for determination of metoprolol in the patients with hypertension

YILMAZ B., ARSLAN S., AKBA V.

TALANTA, cilt.80, sa.1, ss.346-351, 2009 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 80 Sayı: 1
  • Basım Tarihi: 2009
  • Doi Numarası: 10.1016/j.talanta.2009.06.079
  • Dergi Adı: TALANTA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.346-351
  • Anahtar Kelimeler: Metoprolol, GC-MS, Plasma, Method validation, SOLID-PHASE EXTRACTION, BETA-BLOCKERS, HUMAN PLASMA, HUMAN URINE, METABOLITES, QUANTIFICATION, ASSAY, PROPRANOLOL, ENANTIOMERS, VALIDATION
  • Atatürk Üniversitesi Adresli: Evet

Özet

A sensitive and efficient method was developed for determination of metoprolol in human plasma by gas chromatography-mass spectrometry (GC-MS). Metoprolol and atenolol (internal standard, IS) were extracted from human plasma with a mixture of ethylacetate and diethylether at basic pH with liquid-liquid extraction. Calibration curves were linear over the concentration range 15-500 ng/ml. Intra- and inter-day precision values for metoprolol in human plasma were less than 6.4, and accuracy (relative error) was better than 8.8%. The analytical recovery of metoprolol from human plasma averaged 91.20%. The limits of detection (LOD) and quantification (LOQ) of metoprolol were 5.0 and 15 ng/ml, respectively. Also the developed and validated GC-MS method was successfully applied to three patients with hypertension who had been given an oral tablet of 100 mg metoprolol. (C) 2009 Elsevier B.V. All rights reserved.