Efficient genome editing by CRISPR-Cas12a in common wheat via shoot apical meristem delivery
Plant physiology, cilt.199, sa.4, 2025 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 199 Sayı: 4
- Basım Tarihi: 2025
- Doi Numarası: 10.1093/plphys/kiaf611
- Dergi Adı: Plant physiology
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, Chemical Abstracts Core
- Atatürk Üniversitesi Adresli: Evet
Özet
The CRISPR-Cas12a system has emerged as a powerful genome editing (GE) tool, particularly suited for targets with T-rich (TTTV or TTV) protospacer adjacent motifs (PAMs). While Cas12a has been successfully applied in a variety of plant species, including Arabidopsis thaliana, rice (Oryza sativa), soybean (Glycine max), and tobacco (Nicotiana benthamiana), its efficiency in common wheat (Triticum aestivum) remains limited. A prior study reported low Cas12a-mediated GE efficiency in wheat using Agrobacterium-mediated transformation, which has hindered broader adoption of the system in wheat. To overcome the limitation of conventional tissue culture-based transformation methods, a Shoot Apical Meristem (SAM)-based biolistic delivery method has been developed for genotype independent CRISPR-Cas9 mediated GE in wheat. The sub-epidermal (L2) cells of SAM maintain the potential to develop into germline cells, therefore mutations that occur in L2 cells can lead to stably edited plants in the next generation. In this study, to augment CRISPR-Cas12a applications in wheat, we investigated factors that could improve Cas12a-mediated GE efficiency in wheat using the SAM-based delivery approach.