Akademik Veri Yönetim Sistemi
Second international congress on biological and health science, Afyonkarahisar, Türkiye, 24 - 27 Şubat 2022, ss.204-208
Acetylcholinesterase (AChE) hydrolyzes acetylcholine, a neurotransmitter, into acetic acid and choline. AChE activity enhances in the age-related neurological disorders. Thus, the inhibition of AChE is a crucial therapeutic approach to treat the cognitive disorder such as Alzheimer's disease. Natural compounds derived from plants are widely investigated as sources of AChE inhibitors. It has been reported that phenolic compounds naturally found in plants and fruits have the potential to inhibit AChE activity. Gallic acid (GA) and vanillic acid (VA) are phenolic acid derivatives found in plants. These compounds are bioactive molecules with anti-inflammatory, antioxidant, antibacterial and antimicrobial effects. In this study, the effect of different doses of GA and VA mixture on AChE activity was investigated. A mixture of GA and VA was prepared at 50 and 100 μM concentrations of both compounds. This mixture was applied to third instar fly larvae. After the application, the heads of adult flies were dissected and homogenized. AChE activity was measured by Ellman's method for each group. When the results were examined, it was found that 50 μM of GA-VA mixture did not show a significant difference in enzyme activity, but 100 μM of GA-VA mixture caused a significant decrease in enzyme activity. It could be beneficial to use the synergistic effect of phenolic compounds in the investigation of AChE inhibitors.