An Overview on the Mechanisms and Applications of Enzyme Inhibition-Based Methods for Determination of Organophosphate and Carbamate Pesticides

Cao J., Wang M., Yu H., She Y., Cao Z., Ye J., ...Daha Fazla

Journal of Agricultural and Food Chemistry, cilt.68, sa.28, ss.7298-7315, 2020 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Derleme
  • Cilt numarası: 68 Sayı: 28
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1021/acs.jafc.0c01962
  • Dergi Adı: Journal of Agricultural and Food Chemistry
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Analytical Abstracts, Applied Science & Technology Source, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Chimica, Compendex, EMBASE, Environment Index, Food Science & Technology Abstracts, MEDLINE, Pollution Abstracts, Veterinary Science Database, DIALNET
  • Sayfa Sayıları: ss.7298-7315
  • Anahtar Kelimeler: organophosphate pesticides, carbamate pesticides, rapid detection methods, devices, enzyme sources, reactivation, identification, HIGHLY SENSITIVE DETECTION, ACETYLCHOLINESTERASE BASED BIOSENSOR, ON-A-CHIP, ALKALINE-PHOSPHATASE, CRYSTAL-STRUCTURE, RAPID DETECTION, ULTRASENSITIVE DETECTION, AFFINITY-CHROMATOGRAPHY, AMPEROMETRIC DETECTION, COLORIMETRIC DETECTION
  • Atatürk Üniversitesi Adresli: Evet

Özet

Copyright © 2020 American Chemical Society.Acetylcholinesterase inactivating compounds, such as organophosphate (OP) and carbamate (CM) pesticides, are widely used in agriculture to ensure sustainable production of food and feed. As a consequence of their applications, they would result in neurotoxicity, even death. In this essence, the development of enzyme inhibition methods still shows great significance as rapid detection techniques for on-site large-scale screening of OPs and CMs. Initially, mechanisms and applications of various enzyme-inhibition-based methods and devices, including optical colorimetric assay, fluorometric assays, electrochemical biosensors, rapid test card, and microfluidic device, are highlighted in the present overview. Further, to enhance the enzyme sensitivity for detection; alternative enzyme sources or high yield enrichment methods (such as abzyme, artificial enzyme, and recombinant enzyme), as well as enzyme reactivation and identification, are also addressed in this comprehensive overview.