Amylase of moderate thermophilic Niallia alba BT-5: Production, purification, characterization and industrial use potential
International Journal of Biological Macromolecules, vol.376, 2026 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 376
- Publication Date: 2026
- Doi Number: 10.1016/j.ijbiomac.2026.153203
- Journal Name: International Journal of Biological Macromolecules
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, Chemical Abstracts Core, Compendex, EMBASE, INSPEC, MEDLINE
- Keywords: Amylase, Bacteria, Starch affinity technique
- Ataturk University Affiliated: Yes
Abstract
This study aimed to (1) isolate amylase-producing bacteria from different hot springs, (2) purify and characterize the amylase of the highest-potency isolate, and (3) investigate its potential for industrial use. BT-5 was determined as the isolate having the highest amylase activity among nine bacterial isolates. This isolate was identified as Niallia alba. The most suitable culture conditions for amylase production from BT-5 were an initial pH of 9.0, a temperature of 45 °C, a starch concentration of 1.5%, and an incubation time of 48 h. Under these optimized culture conditions, 113.17 U/mL amylase could be produced. Amylase, with the specific activity of 4679.2 U/mg protein, was purified 5.23-fold and obtained with 56.7% yield. The molecular weight of N. alba BT-5 amylase was found to be approximately 72 kDa by SDS-PAGE. Kinetic values (Km and Vmax) of amylase were determined to be 1.189 mg/mL and 1209 U/mL, respectively. The potential of purified amylase for industrial use was investigated. The desizing percentage of the enzyme in textile applications was found to be 3.47%, while the clarification percentage in fresh apple juice was determined to be 37.5% and 56.79% in the presence of 5–10 mM CaCl2, respectively. Furthermore, high stain removal performance was shown when the enzyme was used in combination with the compatible detergent. This study is the first to investigate Niallia alba as a source of amylase, and it reveals that the enzyme has potential for use, particularly in the detergent and beverage industries.