Determination and Pharmacokinetics of Recombinant Human Growth Hormone in Rabbit Plasma by Spectrofluorometry Method

YILMAZ B., KADIOĞLU Y., Meral K., ÇELEBİ F., ONGANER Y.

KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI, cilt.18, sa.4, ss.589-594, 2012 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 18 Sayı: 4
  • Basım Tarihi: 2012
  • Dergi Adı: KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.589-594
  • Atatürk Üniversitesi Adresli: Evet

Özet

In this study, a new spectrofluorometry method was developed for the determination of human growth hormone (somatotropin) in rabbit plasma. The validation was assessed under a variety of conditions (FDA, Bioanalytical Method Validation Guidline). Calibration curves were linear between the concentration range of 0.125-8.0 mu g/mL. Intra- and inter-day precision values for somatotropin in plasma were less than 13.5, and accuracy (relative error) was better than 12.%. The recoveries for all samples were >84.0%. The limits of detection (LOD) and quantification (LOQ) of somatotropin were 0.075 and 0.10 mu g/mL, respectively. The described spectrofluorometry method has adequate sensitivity and specificity to study pharmacokinetics of somatotropin in rabbits, and could be adapted also to clinical pharmacokinetic study.