This research was implemented to develop an achievement test toward “Reproduction, Growth and Development in Plants and Animals” subject. The data of this quantitative research were collected through survey design methodology. In the test development process, the literature was firstly reviewed and then 30 multiple choice questions in accordance with learning outcomes in the science course curriculum was determined. For the validity study, the questions in test were presented to the opinion of academicians and teachers who are experts in their fields. After expert opinions, questions were examined in terms of language, scope, understandability, etc., and corrections were made in terms of spelling and content when necessary. Finally, the number of questions was reduced to 20 by taking the age group into account. The draft test with 20 questions was applied to 58 students who attended the sixth grade of a secondary school in the city center of Erzurum. Subsequently, item analysis was performed on the data obtained from 55 students (29 girls, 26 boys) who were evaluated. In the item analysis, the average difficulty of the test was calculated as 0.66 and the average discriminant was 0.56. In addition, some questions that were thought to remain in the test were corrected. After the item analysis, the reliability studies of the test was performed with a different group of 60 students. According to the reliability analysis, the KR-20 reliability coefficient for achievement test was 0.93. As a result of the validity and reliability analysis, it was seen that the final test was a valid and reliable test that includes questions at different difficulty levels of Bloom’s Taxonomy.