Glucose 6-phosphate dehydrogenase: In vitro and in vivo effects of dantrolene sodium

BEYDEMIR S., Gulcin I., Kufrevioglu O., CIFTCI M.

POLISH JOURNAL OF PHARMACOLOGY, cilt.55, sa.5, ss.787-792, 2003 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 55 Sayı: 5
  • Basım Tarihi: 2003
  • Dergi Adı: POLISH JOURNAL OF PHARMACOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.787-792
  • Anahtar Kelimeler: glucose 6-phosphate dehydrogenase, erythrocytes, human, dantrolene sodium, CARBONIC-ANHYDRASE, ENZYME-ACTIVITIES, ERYTHROCYTES, PURIFICATION, DEHYDROGENASE, ANTIBIOTICS, DEFICIENCY, SULFATE, BLOCKS, MUSCLE
  • Atatürk Üniversitesi Adresli: Evet

Özet

In our study, effects of dantrolene sodium on glucose 6-phosphate dehydrogenase (GOD) were examined in the human erythrocytes in vitro and in rat erythrocytes in vivo. Human erythrocyte GOD was purified using ammonium sulfate fractionation and 2',5'-ADP Sepharose 4B affinity chromatography. The enzyme activity was determined by Beutler's method. The overall purification procedures gave the human GOD having the specific activity of 97.6 EU/mg of protein, which was purified 9760-fold with a yield of 39%. Dantrolene sodium inhibited the enzyme activity under in vitro conditions and the 150 value (drug concentration which produces 50% inhibition) of this drug was 0.91 mM. In vivo studies were performed in rats (Sprague-Dawley). Dantrolene, sodium at 10 mg/kg inhibited the enzyme activity significantly (p < 0.05) 3 h after dosing. We conclude that dantrolene sodium showed inhibitory effect on GOD activity both in vitro and in vivo.