Akademik Veri Yönetim Sistemi
ChemistrySelect, cilt.11, sa.9, 2026 (SCI-Expanded, Scopus)
This study investigates phenazine-1-carboxylic acid (PCA) production by Pseudomonas chlororaphis subsp. aureofaciens under submerged batch fermentation. This nonpathogenic, flexible carbon-utilizing strain was selected as a safer and industrially adaptable PCA producer. Chemical and physical parameters were optimized, and phenazines were isolated by chloroform extraction followed by adsorption chromatography and characterized using UV–vis, FTIR, and 1H-NMR analyses. PCA production was identified as growth-associated, yielding 129.45 µg/mL—comparable to wild-type Pseudomonas strains despite the use of low-cost glycerol medium-highlighting the process's industrial relevance. Key kinetic parameters were determined as YXS = 0.64 mg/mg, YPS = 0.12 mg/mg, YPX = 0.35 mg/mg, and μ = 0.39 h−1. PCA exhibited antimicrobial activity against Klebsiella pneumoniae (10 mm) and Micrococcus luteus (22 mm) at 150 µg/mL. Cytotoxicity was evaluated via MTT assay on A549 and SKOV3 cells, yielding IC50 values of 17.30 ± 1.72, 13.04 ± 0.76, and 5.69 ± 0.35 µg/mL at 24, 48, and 72 h, respectively, with notable effects also observed in SKOV3 cells. Overall, the findings demonstrate that PCA possesses significant antimicrobial and anticancer potential, supporting its further development as a natural bioactive compound.