Kinetic analysis of multi enzyme systems: A case study of the closed system of creatine kinase, hexokinase and glucose 6-phosphate dehydrogenase

YILDIRIM N., CIFTCI M., Kufrevioglu O.

JOURNAL OF MATHEMATICAL CHEMISTRY, cilt.31, sa.1, ss.121-130, 2002 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 31 Sayı: 1
  • Basım Tarihi: 2002
  • Doi Numarası: 10.1023/a:1015490531653
  • Dergi Adı: JOURNAL OF MATHEMATICAL CHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.121-130
  • Anahtar Kelimeler: symbolic and numerical computation, kinetics of multi enzyme system, steady state assumption, parameter estimation
  • Atatürk Üniversitesi Adresli: Evet

Özet

This paper describes a general methodology to handle closed multi enzyme systems using mixture of symbolic (which depends on the Grobner Basis technique) and numerical computation methods. The applicability of the proposed method has been examined for the closed three-enzyme system of rabbit heart creatine kinase (EC 2.7.3.2), yeast hexokinase (EC 2.7.1.1) and human erythrocyte glucose 6-phosphate dehydrogenase (EC 1.1.1.49) using experimental data.