Akademik Veri Yönetim Sistemi
2. Uluslararası ve 27. Ulusal Farmakoloji Kongresi / 2nd International and 27th National Pharmacology Congress, Antalya, Türkiye, 23 - 26 Kasım 2023, ss.191-192
Objective: The aim of this study was to investigate the effects of bosentan, an endothelin receptor blocker, on wound healing in L929 fibroblast cells. Method: L929 cell line obtained from American Type Culture Collection (ATCC, USA) and stored in Cryotube, were removed from the liquid nitrogen tank, and seeded in a T75 cm2 flask containing DMEM medium containing 10% FBS and incubated at 37°C, 90% humidity and 5% CO2. L929 mouse fibroblast cells were seeded with 5000 cells per well of a 96-well plate. The effect of bosentan at concentrations of 10-3, 10-4 ,10-5 , 10-6 , 10-7, 10-8 , 10-9 M on cell viability was evaluated at 24, 48 and 72 hours using MTT assay. Then, using the in vitro scratch wound healing test, the effects of bosentan concentrations (10-5 , 10-6 , 10-7 M), which gave the best results in the cell viability test, on wound healing at 12, 24, 36 hours were examined. Images of the wells were taken at 0, 12, 24 and 36 hours. At the end of experiment all groups supernatants were collected and TGF-β1 levels were analyses with ELISA. IBM SPSS 20 was used for statistical analyses. Results: MTT results showed that 10-3concentration of bosentan decreased cell viability. 10-5 , 10-6 , 10-7 M concentrations of bosentan significantly increased cell proliferation compared to control and other drug groups. Bosentan concentrations of 10-5 and 10-6 M and 10-7 M were observed to increase the migration of fibroblast cells to the injured area when compared with the control group by percentage closure calculation. When the TGF-β1 results were analyzed, it was observed that there was a significant decrease in the TGF-β1 amounts of the drug groups compared to the control group. Conclusion: In the scratch wound healing test, especially the 10-5 M concentration of bosentan showed near complete closure compared to the control and other groups. As a result, bosentan triggers the migration of fibroblasts, which have an active role in the wound healing process, to the wound area and provides wound closure. Keywords: Bosentan, Wound healing, Scratch wound healing assay, L929 cell line