Rapid colorimetric determination of the pesticides carbofuran and dichlorvos by exploiting their inhibitory effect on the aggregation of peroxidase-mimicking platinum nanoparticles

Cao J., Wang M., She Y., Abd El-Aty A. M., HACIMÜFTÜOĞLU A., Wang J., ...Daha Fazla

MICROCHIMICA ACTA, cilt.186, sa.6, 2019 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 186 Sayı: 6
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1007/s00604-019-3485-7
  • Dergi Adı: MICROCHIMICA ACTA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Anahtar Kelimeler: Organophosphates, Carbamates, Platinum nanoparticles, Enzyme inhibition, Colorimetric assay, Enzyme mimic, ORGANOPHOSPHORUS PESTICIDE, SENSITIVE DETECTION, ACETYLCHOLINESTERASE, RESIDUES, APTASENSOR
  • Atatürk Üniversitesi Adresli: Evet

Özet

A novel and highly sensitive enzyme inhibition assay was developed for the rapid detection of the organophosphate pesticide dichlorvos and the carbamate pesticide carbofuran. It achieves signal amplification by the secondary catalysis of platinum nanoparticles. Acetylcholinesterase (AChE) is capable of catalyzing the hydrolysis of acetylthiocholine to form thiocholine. Thiocholine causes the aggregation of citrate-capped platinum nanoparticles which then lose their peroxidase-mimicking properties. After addition of pesticides, the activity of AChE is inhibited, less thiocholine is produced, less aggregation occurs, and the peroxidase-mimetic properties are increasingly retained. In the presence of tetramethylbenzidine and H2O2, a deep blue coloration with an absorption maximum at 650 nm will be formed. The assay was applied to the determination of dichlorvos and carbofuran, and detection limits of 2.3 mu g.L-1 and 1.4 mu g.L-1 were obtained, respectively. Recovery experiments with spiked tap water and pears gave satisfactory relative standard deviations.